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. 2020 Nov 12;7:574730. doi: 10.3389/fnut.2020.574730

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Copyright © 2020 Lawton, Morgan, Schreiner, Schreiner, Baumann, Upchurch, Xu, Price and Isaacs.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Folate-dependent genes are not ubiquitously expressed. Each of the genes confirmed to be differentially expressed in hippocampus was also tested in liver and heart tissue of the same mice to determine if differential expression was systemic or tissue specific. Each gene was tested on 3 independent biological replicates in the FA and deficient (DEF) conditions. (A) Of the 15 genes tested, only one was found to be differentially expressed in the liver (Pacrgl), and no genes were found to be differentially expressed in the heart. The blue dotted line represents a significant P-value (≤0.05). (B) Interestingly, only one of the differentially expressed hippocampus genes was also differentially expressed in the liver (Pacrgl, hippocampus P = 0.05, liver P = 0.04; significance noted by asterisks). Further, Pacrgl was significantly downregulated in the hippocampus and significantly upregulated in the liver in the deficient vs. folate comparison. This same gene was not differentially expressed in the heart (P = 0.43). Error bars represent SEM values calculated from the 3-fold values prepared from three independent biological replicates.