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. 2020 Sep 22;29(19):3224–3248. doi: 10.1093/hmg/ddaa209

Figure 5.

Figure 5

Primary microglia from Trem2 R47H KI mice show decreased Trem2 expression, and an impaired IL-4-induced anti-inflammatory response. (A) Expression of Trem2. (B) Expression of anti-inflammatory markers, Arg1 and Tgf1b. (C) Expression of other microglial genes. Gene expression was normalized to Rps28 and calculated as fold change relative to the WT control without IL-4 treatment. N = 8–15 mice per genotype. Data shown as mean ± SEM. Two-way ANOVA (for all genes except Arg1). Significant main effect of IL-4-treatment and genotype indicated as horizontal and vertical lines respectively, no significant interactions were seen between IL-4-treatment and Trem2 knockdown. Sidak’s post hoc tests were performed to test pairwise significance between the three genotypes within a treatment group. Arg1 was analyzed by one-way ANOVA (P = 0.002) followed by Sidak’s multiple comparisons to test pairwise significance between the three genotypes within a treatment group. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.