Fig. 7.

Chemotherapy and belinostat reprogrammed transcriptome of patient cells. Unbiased UMAPs for ER+ patient sample Patient#6 (A) and Patient#5 (B) under treatment (Tx). Patient cells were cultured in Renaissance medium (3D) and treated with chemotherapy (doxorubicin 0.1 µM, carboplatin 0.1 mM, paclitaxel 1 µM) for 72 h, and then treaded with DMSO/belinostat (1 µM) for 72 h. The cells were collected, purified with Dead Cell Removal Kit, and subjected to ICELL8^Ⓡ Single-Cell System as described in Methods. For each patient, the UMAPs for all treatments were shown in the top, and the UMAPs for each individual treatment were shown in the bottom with different colors indicated as the legends. Violin plots with GSEA pathways enrichment scores related to HDAC targets, stem cell differentiation and MYC pathway were presented in (C) for Patient#6 and (D) for Patient#5. The statistical analysis was performed with student's t-test between the indicated groups, and significance is marked with * for P<0.05, ** for P<0.01, *** for P<0.001, **** for P<0.0001, *****for P<0.00001.