Figure 3.

GJA1 Acted as a Key Downstream Target of TSPEAR-AS2 in GC Progression

(A) Heatmap of altered genes in GC cells transfected with TSPEAR-AS2 siRNA or Scrambled. (B) Hierarchical clustering gene transcription altered in GC cells after knockdown of TSPEAR-AS2. (C) The qRT-PCR assays were conducted to validate the level of key genes in GC cells with TSPEAR-AS2 knockdown. (D) Gene Ontology analysis for all genes with altered expressions after knockdown of TSPEAR-AS2. (E) GSEA explored the gene sets enriched by genes in response to TSPEAR-AS2 knockdown (Negative regulation of growth). (F) GSEA explored the gene sets enriched by genes in response to TSPEAR-AS2 knockdown (Positive regulation of extrinsic apoptotic signaling pathway). (G) The expression of GJA1 was determined in GC cells treated with TSPEAR-AS2 siRNA or scrambled using qRT-PCR assays. (H) The expression of GJA1 was determined in GC cells treated with TSPEAR-AS2 siRNA or scrambled using western blot assays. *p < 0.05, **p < 0.01.