Figure 5.

Regulation Relationship between lncRNA TSPEAR-AS2 and miR-1207-5p in GC

(A) Bioinformatics databases (miRanda, pita, and RNAhybrid) were analyzed to predict potential miRNAs binding with TSPEAR-AS2. (B) Luciferase activity of HEK293T cells cotransfected with 4 various miRNA-coding plasmids and the luciferase reporter plasmids (pmirGLO-TSPEAR-AS2-WT). Data are presented as the ratio of firefly luciferase to Renilla luciferase activity. (C) Luciferase activity in HEK293T cells cotransfected with miR-1207-5p or negative control and pmirGLO-TSPEAR-AS2-WT or pmirGLO-TSPEAR-AS2-Mut. (D) The level of miR-1207-5p was examined in GC cells with TSPEAR-AS2 knockdown or overexpression through qRT-PCR assays. (E) RNA levels in immunoprecipitates were presented as fold change in Ago2 relative to IgG immunoprecipitates. (F) Relative expression of miR-1207-5p in GC tissues and paired normal tissues was analyzed in the GSE54397 database. (G) The level of miR-1207-5p was detected in GC cells transfected with mimic or inhibitor against miR-1207-5p using qRT-PCR assays. (H and I) The effects of miR-1207-5p on GC cell apoptosis were analyzed by flow cytometry assays. (J) The effects of miR-1207-5p knockdown or overexpression on GC cell viability. ∗p < 0.05, ∗∗p < 0.01.