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. 2020 Nov 25;10:20563. doi: 10.1038/s41598-020-77521-0

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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(A) Ag cellular burden of P. malhamensis exposed to 1.0 mg L⁻¹ AgNPs or 40.7 μg L⁻¹ AgNO₃ during 2 h and 24 h. The results are presented as ng of cellular Ag per 1 × 10⁷ cells. Asterisk indicates a significant difference between treatments obtained by two-way analysis of variance (ANOVA) followed by a Sidak’s multiple comparisons test (p < 0.05); (B,C) TEM images of P. malhamensis: treated with 1 mg L⁻¹ AgNPs for 2 h (B) and for 24 h (C); (D,E) energy dispersive X-ray (EDX) spectra correspond to the interest area from C (D) and D (E). Letters on (B) denote cell organelles: G Golgi body, M mitochondrion, N nucleus, FV food vacuole, L lipid drops, CP chloroplast. *p < 0.05, **p < 0.01, ***p < 0.001.