Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Nov 25;11:5982. doi: 10.1038/s41467-020-19657-1

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 1 — a–c Beta cell replication of 20wo mouse islet grafts 12-days after implantation into the anterior chamber of the eye of p16 or 20wo C57BL6/J recipients. a Representative images of islet grafts co-immunostained for insulin (purple) /ki67 (green) or insulin (purple)/pHH3 (green). Nuclei are marked with Hoechst in blue. b Quantification of the percentage of beta (insulin+) cells that are ki67+ in islet grafts transplanted into p16 (n = 11, blue) and 20wo (n = 5, purple) mice. c Quantification of the percentage of beta (insulin+) cells that are pHH3+ in islet grafts transplanted into p16 (n = 4, blue) and 20wo (n = 3, purple) mice. d–f Beta cell replication of adult human islet grafts 12-days after implantation into the anterior chamber of the eye of p16 or 20wo NSG-SCID mouse recipients (islets from two donors). d Representative images of islet grafts co-immunostained for insulin (purple)/ki67 (green) or insulin (purple)/pHH3 (green). Nuclei are marked with Hoechst in blue. e, f Quantification of the percentage of beta (insulin+) cells that are ki67+ (e) or pHH3+ (f) in human islet grafts transplanted into p16 (n = 5, green) or 20wo (n = 4, yellow) mice. Data shown represent mean ± SEM for the indicated n. *p < 0.05; **p < 0.01, using two-tailed Student’s t test. Scale bars are 25 μm.