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. 2020 Nov 25;11:5982. doi: 10.1038/s41467-020-19657-1

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a Representative images of antibody arrays incubated with p14 or 20wo mouse serum. Spots corresponding to Wisp1 are marked with an empty square box. b Serum levels of Wisp1 in p14 (n = 7), p28 (n = 4), 11wo (n = 6) and 20wo (n = 7) mice measured with ELISA. c Plasma levels of WISP1 in children (n = 11) and adult (n = 14) measured with ELISA. d Quantification by qPCR of the expression of the indicated CCN genes in p14 (n = 7, green) and 20wo mouse islets (n = 6 for Cyr61, n = 7 for other genes, gray). Values are expressed relative to Tbp. e Quantification by qPCR of the expression of the indicated CCN genes in adult human islets (n = 6 for CYR61, n = 5 for other genes). Values are expressed relative to TBP. f Quantification by qPCR of Wisp1 gene expression in the indicated p14 (green) and 20wo (gray) mouse tissues. Wisp1 gene expression is shown relative to levels in p14 bone, given the value of 1 (n = 9 for bone, n = 4 for all other tissues). WAT: white adipose tissue; Gastroc: gastrocnemius. All data shown represent mean ± SEM from the indicated n. Indicated comparisons were made using two-tailed Student’s t test (c, d), one-way (b) and two-way (f) ANOVA. *p < 0.05; **p < 0.01; ****p < 0.0001; ns: not significant. In f, at p14, bone Wisp1 gene expression was significantly higher than in all other tissues tested with p < 0.01–0.001.