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. 2020 Nov 25;11:5982. doi: 10.1038/s41467-020-19657-1

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a–c Beta cell proliferation in fixed pancreases from p14 Wisp1⁺/⁺ or Wisp1⁻/⁻ mice. a Representative images of pancreases co-immunostained for insulin (purple)/ki67 (green) or insulin (purple) /pHH3 (green). Nuclei are marked with Hoechst in blue. b Quantification of the percentage of beta (insulin+) cells that are ki67+ in Wisp1⁺/⁺ (n = 5, yellow) or Wisp1⁻/⁻ (n = 6, orange) mice. c Quantification of the percentage of beta (insulin+) cells that are pHH3+ in Wisp1⁺/⁺ (n = 4, yellow) or Wisp1⁻/⁻ (n = 5, orange) mice. d–f Beta cell proliferation in fixed pancreases from p12 Wisp1⁻/⁻ mice treated with saline or with rmWisp1 protein for three days (from p9 to p11). d Representative images of pancreases co-immunostained for insulin (purple)/ki67 (green) or insulin (purple)/pHH3 (green). Nuclei are marked with Hoechst in blue. e Quantification of the percentage of beta (insulin+) cells that are ki67+ in mice injected with rmWisp1 (n = 4, orange) o saline (n = 4, brown). f Quantification of the percentage of beta (insulin+) cells that are pHH3+ in mice injected with rmWisp1 (n = 3, orange) o saline (n = 3, brown). g–i Beta cell proliferation of 20wo mouse islet grafts transplanted into the anterior chamber of the eye of p16 Wisp1⁺/⁺ or Wisp1⁻/⁻ mouse recipients. g Representative images of islet grafts co-immunostained for insulin (purple)/ki67 (green) or insulin (purple)/pHH3 (green). Nuclei are marked with Hoechst in blue. h Quantification of the percentage of beta (insulin+) cells that are ki67+ in p16 Wisp1⁺/⁺ (n = 7, yellow) or Wisp1⁻/⁻ (n = 7, orange) mice. i Quantification of the percentage of beta (insulin+) cells that are pHH3+ in p16 Wisp1⁺/⁺ (n = 4, yellow) or Wisp1⁻/⁻ (n = 4, orange) mice. All data values represent mean ± SEM for the indicated n. *p < 0.05; **p < 0.01 using two-tailed Student’s t test. Scale bars are 25 μm. ND: not detectable.