Fig. 2. cCNTNAP3 inhibits Eca-109 cell growth and induces cell cycle arrest and apoptosis in vitro.

a cCNTNAP3 expression in HEEC and multiple ESCC cell lines was measured using qRT-PCR. b–d RTCA, colony formation, and EdU assays of Eca-109 cells with cCNTNAP3 knockdown or overexpression. e, f Cell cycle and cell apoptosis rate of Eca-109 cells that received the indicated treatments are analyzed by FACS. All the results were shown as mean ± SD (n = 3), which were three separate experiments performed in triplicate. *p < 0.05, **p < 0.01, ***p < 0.001 (Student’s t test).