Fig. 5. miR-513a-5p reverses the tumor-suppressor roles of cCNTNAP3 in Eca-109 cells.
a The expression level of miR-513a-5p in Eca-109 cells was analyzed by qRT-PCR. b The transfection efficiency of miR-513a-5p inhibitor or mimic was determined by qRT-PCR in Eca-109 cells. c–e RTCA, colony formation, and EdU assays were performed in Eca-109 cells after cCNTNAP3 knockdown or overexpression and cotransfection with miR-513a-5p inhibitor or mimic. All the results were shown as mean ± SD (n = 3), which were three separate experiments performed in triplicate. *p < 0.05, **p < 0.01, ***p < 0.001 (Student’s t test).