Fig. 1. Manhattan plot displaying results from the RLS discovery meta-analysis for N = 480,982 independent biological samples.

Variants labeled orange are previously reported variants. Variants labeled blue and green are novel variants (five) that were tested in a follow-up sample. Of the five novel variants, three were confirmed (green diamond shape) in the follow up analysis and met the genome-wide significance threshold^27,28, whereas two did not (Table 1). (see Supplementary Table 1 for details; See Supplementary Figs. 1–5 for regional Manhattan plots displaying the five novel RLS-associated variants).