Fig. 4.
Dicerflox/flox mice were treated with AAV8 vector expressing or not expressing Cre recombinase (Cre or null). a) Western blot of kidney cortex CYP2E1 A, C, E. mice treated with AAV8 null then kidney collected 1, 2 or 3 weeks later. B, D, F mice treated with AAV8 Cre then kidney collected 1, 2 or 3 weeks later b) Cytochrome P450 2E1 (CYP2E1) mRNA expression is expressed as b-actin(ct) - CYP2E1 (ct). 3 weeks after AAV8 treatment mice received paracetamol 150 or 300 mg/kg (or vehicle (0)) ip, then the kidney cortex was harvested 6 hours later. c). CYP2E1 activity was determined by metabolism of chlorzoxazone.d) Kidney injury molecule 1 (KIM-1) mRNA expression in the kidney cortex following paracetamol (300mg/kg ip) in no AAV8 treated (untreated), AAV8 null and AAV8 Cre treated mice. KIM-1 is normalized by beta-actin. For a-d, statistical significance was determined by Mann-Whitney Test. Data are represented individual mice with bars representing median and IQR (N=5 per group).