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. 2020 Nov 25;10:20583. doi: 10.1038/s41598-020-76541-0

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Bodyweight changes of BALB/c mice infected with the H6 AIVs. 4-week-old female BALB/c mice were inoculated i.n. with 10^6.0EID₅₀ of virus in a volume of 30.0 μl. The body weight of 5 mice were measured daily until 14 dpi. Weight changes of the BALB/c mice infected with H6N2 (Fig. 3a) and H6N6 (Fig. 3b) were shown respectively. The data were graphed using the Prism 6.0 software (Vision 6.0, GraphPad Prism). Comparisons of the weight changes between two groups were determined by nonparametric t-tests using GraphPad. (*P < 0.05, **P < 0.01, ***P < 0.001).