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. 2020 Nov 20;14(1):100947. doi: 10.1016/j.tranon.2020.100947

Fig. 4.

Fig. 4

LncRNA LINC00355 promotes expression of ITGA2 via recruiting GTF2B. A, Correlation analysis of GTF2B and ITGA2 in colon cancer. The abscissa represents ITGA2 expression while the ordinate represents GTF2B expression. Each point shows one sample. Correlation coefficient and correlation p value are listed above. B, Localization of lncRNA LINC00355 in cells by FISH; C, Luciferase signal of lncRNA LINC00355 in COLO205 cells detected by dual luciferase reporter gene assay; D, lncRNA LINC00355 expression in COLO205 cells detected by RIP test; E, relative expression of ITGA2 promoter determined by CHIP assay; F, Expression of ITGA2 in COLO205 cells measured by Western blot assay. G, ITGA2 protein expression detected by Western blot analysis. * p < 0.05 vs. cells infected with NC + ITGA2 prom WT, IgG, blank, or pLV-EGFP-N + sh-NC. # p < 0.05 vs. pLV-EGFP-N-LINC00355 + sh-NC. Data were expressed as mean ± standard deviation. Data were in compliance with normal distribution and homogeneity between two groups and were compared using an unpaired t-test. Data comparison between groups was performed using one-way ANOVA with Tukey's post hoc test.