Figure 2.

A low dose of the CD19-specific BsAb redirects polyclonal T cells to treat ALL xenografts in vivo. (A–C) Immunodeficient NSG mice were intravenously inoculated with CD19(+) NALM6-luciferase human ALL xenografts (0.5×10⁶ cells). Therapy was started after 3 days. Mice received weekly injections of 10⁷ ATC with different doses of BC250. In the control groups, mice either received ATCs or a control GD2 BsAb with ATCs. Leukemia growth was monitored using the IVIS bioluminescent imager (A–B) and survival was assessed over time. Area under the curve of the log total flux until day 17 was compared between groups using ANOVA and multiple comparison analysis (C). All mice received subcutaneous injections of interleukin-2 (1000 IU/two times/week). The experiment with NALM6 cells was repeated two times. ALL, acute lymphoblastic leukemia; ATC, activated human Tcells; BsAb, bispecific antibody; ANOVA, analysis of variance.