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. 2020 Nov 25;40(11):BSR20201545. doi: 10.1042/BSR20201545

Figure 3. miR-200a-3p negatively regulated TGF-β2 expression.

Figure 3

(A) Bioinformatics analysis indicated the putative binding sites and corresponding mutant region for miR-200a-3p within TGF-β2. (B) Dual-luciferase reporter gene assay for WT and MUT TGF-β2 3′UTR luciferase vectors in HEK-293T cells transfected with control or miR-200a-3p mimic. (C) The expression of TGF-β2 protein in ARPE-19 cells transfected with miR-200a-3p mimic was determined by Western blotting. **P<0.01, compared with the NC group.