Figure 1.

Effect of conservation. (A) Relationship between conservation and fitness was studied using the homologous sequences for TEM-1 β-lactamase (Pfam ID PF13354). It can be seen that the number of neutral substitutions decreases considerably for amino acids with conservation >60%. In the box-plot representation, the black filled circle and the red line represent the mean and median of the fitness, respectively. The whiskers are plotted at the lowest data point greater than Q1 − 1.5 × (Q3 − Q1) and the greatest data point less than Q3 + 1.5 × (Q3 − Q1) where Q1 and Q3 represent the first and the third quartile respectively. Black open circles represent the outliers. (B) Changes in the F1 score for the neutral and deleterious classes and the average of both plotted, as the threshold for conservation to classify the mutations is varied.