Table 1.
Main characteristics of the studied thermophilic DNA polymerases and their TAdUTP‐inccorporating capacity.
|
Polymerase |
3′–5′ Exonuclease activity |
TAdUTP incorporation |
Specifications |
Family |
|---|---|---|---|---|
|
iProof |
yes |
no |
Pyrococcus‐like enzyme with a processivity‐enhancing Sso7d domain |
B |
|
OneTaq |
yes |
no |
mixture of family A and B Taq and Deep Vent, respectively |
A & B |
|
Vent(exo‐) |
no |
yes |
Thermococcus litoralis |
B |
|
PWO Superyield |
yes |
no |
Pyrococcus woesei |
B |
|
Q5U |
yes |
no |
high‐fidelity DNA polymerase fused to a processivity‐enhancing Sso7d domain |
B |
|
KOD XL |
yes/no |
yes |
mixture of KOD and KOD(exo‐) |
B |
|
Pfu |
yes |
no |
Pyrococcus furiosus |
B |
|
Therminator |
no |
no |
9°N exo‐ variant |
B |