Figure 2.

Gamma‐aminobutyric acid (GABA) induction after activation of mitogen‐activated protein kinase (MPK)3/MPK6 in glutamate decarboxylase (gad)1, gad2 and gad4 mutants

(A) Fourteen‐d‐old DD, DD gad1, DD gad2, and DD gad4 seedlings were sprayed with 30 μmol/L dexamethasone (DEX). Samples were collected 24 h later for GABA analysis. One‐way analysis of variance (ANOVA) was performed to compare different genotypes with DD at the same time point (*P < 0.05; ****P < 0.0001). Error bars indicate SD (n = 3), FW, fresh weight. (B) Levels of the Flag‐tagged NtMEK2^DD protein in the different lines were detected by immunoblot analysis using an anti‐Flag antibody.