Fig. 7.

Transcript accumulation of plastid‐encoded RNA polymerase (PEP)‐dependent genes in rice (Oryza sativa) wild‐type (WT) and osppr16 mutant leaves. (a, b) Quantitative real‐time PCR analysis of the expression of PEP‐dependent genes in WT and osppr16 leaves at the (a) three‐leaf stage and (b) five‐leaf stage. OsTPI was used as reference gene. Error bars indicate SD (n = 3); *, P < 0.05; **, P < 0.01 (Student’s t‐test) in (b); all P < 0.01 in (a). (c) Immunoblot analysis of selected proteins encoded by PEP‐dependent genes in WT and osppr16 leaves at the three‐leaf stage. Samples of 25 μg total cellular protein extracted from osppr16d leaves were loaded and compared with a dilution series of WT protein extracts. The heat shock protein HSP82 was used as an internal control. (d) Immunoblot analysis of the same proteins at the five‐leaf stage. Samples of 25 μg total protein from WT and osppr16d leaves were analyzed by Western blot analysis. HSP82 was used as an internal control.