FIGURE 1.

Chemical exposure of MUTZ‐LCs. MUTZ‐LCs were exposed to chemicals or vehicle (water) for 16 hours as described in the “Materials and Methods” section. CD83/CD86 double‐positive cells were quantified by flow cytometry using Flow‐Count fluorospheres and CD83‐PE and CD86‐FITC antibodies; CXCL8 secretion was determined by ELISA (bars). Relative viability compared with vehicle‐exposed MUTZ‐LCs was determined by propidium iodide uptake (flow cytometry; dots). (A) MUTZ‐LC maturation by NiSO₄ and cytokine maturation cocktail (CMC). (B) The percentage of CD83/CD86 double‐positive cells after titanium salt exposure. (C) CXCL8 secretion after titanium salt exposure. The figures depict the average of four individual experiments performed in duplicate ± SEM. *P < .05 calculated using the Friedman multiple comparisons test. FITC, fluorescein isothiocyanate; LC, Langerhans cell; PE, phycoerythrin