Figure 1.

MFG‐E8 gene identification in C57BL/6 mice. Mouse DNA was acquired from the tail and was extracted by a genome DNA kit. The amplification product from the polymerase chain reaction was analyzed via gel electrophoresis imaging to determine the genotype. Both Primer 1 and Primer 5 were used to distinguish between different genotypes. Knockout mice (MFG‐E8^−/−) only expressed the Primer 1 allele, whereas wild‐type mice (MFG‐E8^+/+) only expressed the Primer 5 allele, and the heterozygous mice (MFG‐E8^+/−) expressed both Primer 1 and Primer 5. MFG‐E8, milk fat globule‐EGF factor 8