Fig. 6.

cpm2, a rice AOC mutant, displayed hyposensitivity to abscisic acid (ABA). (a) Germination time courses of the wild‐type (WT) and cpm2 mutants grown on half‐strength Murashige & Skoog (½MS) medium containing different concentrations of ABA (0, 2, 5 μM). (b) The relative germination of the WT and cpm2 seeds under ABA treatments were determined after 4 d and expressed as a percentage of those grown under the ‘mock’ condition. (c) Germination phenotypes of the WT and cpm2 treated with 0, 2, 5 μM ABA. Photographs were taken on day 4. Bars, 1cm. (d) Seedling heights of the WT and cpm2 in accordance with (c). (e) Growth inhibition of seedling height by ABA. The relative growth of the WT and cpm2 seeds under ABA treatments were determined after 4 d and expressed as a percentage of those grown under the ‘mock’ condition. (a, d) Error bars indicate SD with biological triplicates (n = 3, each replicate containing 50 seeds) (a) and 50 biological replicates (n = 50) (d). Asterisks indicate the significance of differences between the WT and transgenic lines as determined by Student’s t‐test analysis in (d) and (e): *, P < 0.05; **, P < 0.01. Oryza sativa ssp. japonica cv Nihonmasari was used as the wild‐type.