Fig. 2.

Silencing of LINC00152 attenuated EMT and EC cell resistance to L-OHP. a–b the expression of LINC00152 after overexpressing or knocking down LINC00152 in Kyse-150 and TE-1 cells detected by RT-qPCR assay. c–d cell survival rate after 72 h of treatment with different concentrations of L-OHP (0, 0.5, 1.0, 2.5, 5.0, 10.0 µM) in Kyse-150 and TE-1 cells detected by CCK-8 assay. e–f cell survival rate after 0, 24, 48 and 72 h of treatment with 10.0 µM of L-OHP in Kyse-150 and TE-1 cells detected by CCK-8 assay. g–n the protein expression of E-cadherin, cleaved PARP and cleaved Caspase 3 in Kyse-150 and TE-1 cells after 72 h of treatment with 10.0 µmol/L of L-OHP determined by Western blot analysis. * p < 0.05 vs. the oe-NC group or the si-NC group. Data in compliance with normal distribution and homogeneity were compared using unpaired t-test. Comparisons among multiple groups were conducted by ANOVA with Tukey’s post hoc test. Data at different time points and different concentrations were compared by repeated measures ANOVA. Data are presented as mean ± standard deviation of three technical replicates