Fig. 3.

B16F10 mouse melanoma cells with AGC1-knockdown have higher potential to metastasize. a Percent of the metastatic areas (%) in the lungs of mice with control (NTC), or AGC1-KD B16F10 cells, measured 16 days after intravenous injection of cells (n = 8). b Representative images of lungs with metastasis harvested 16 days after control (NTC), and AGC1-KD B16F10 cells were intravenously injected. c (left) Number of metastatic tumor loci and (right) average tumor locus size in the lungs of mice with control (NTC), or AGC1-KD B16F10 cells, measured 16 days after intravenous injection of the cells (n = 8). d Representative images of the metastatic areas of mice injected with control (NTC) or AGC1-KD B16F10 tumors, stained with proliferation (PCNA) and apoptosis (cleaved caspase 3; CC3) markers. Images were taken at × 40 magnification. All experiments denote mean ± SDs. Significance levels: * p ≤ 0.05