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. 2020 Nov 26;15:564. doi: 10.1186/s13018-020-02108-4

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 4 — Immunofluorescent staining of Runx-2 and fibronectin expression in osteoblasts. Stained with reds are Runx-2 and fibronectin. TPS-coated surface stained with fibronectin is showed in a and b while fibronectin staining of DMF surface is showed in c and d. Runx-2 expression of TPS surfaces is showed in e and f while that of DMF surface are showed in g and h. Blue stains are of DAPI, which were used as counterstain. Overall expression within the set area is shown in a, c, e, and g. The thickness of the stained layer is shown in b, d, f, and h