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. 2020 Jun 16;11(27):7092–7101. doi: 10.1039/d0sc00339e

Fig. 2. (A) Fluorescence responses of the activable PER based-DNA machine to target mRNA. (a) 200 nM primers + 20 nM survivin DNA + 200 μM dNTPs + 25 U mL–1 KFP + 30 nM MB; (b) 20 nM HP + 20 nM survivin DNA + 200 μM dNTPs + 25 U mL–1 KFP + 30 nM MB; (c) 20 nM HP + 200 nM primers + 20 nM survivin DNA + 25 U mL–1 KFP + 30 nM MB; (d) 20 nM HP + 200 nM primers + 20 nM survivin DNA + 200 μM dNTPs + 30 nM MB; and (e) 20 nM HP + 200 nM primers + 20 nM survivin DNA + 200 μM dNTPs + 25 U mL–1 KFP + 30 nM MB. (B) Corresponding to polyacrylamide gel electrophoresis (PAGE) of the PER based-DNA machine as mentioned above. (C) Relationship between the fluorescence intensities at 665 nm and the concentrations of the survivin DNA target from 0 to 50 nM. Insert: linear correlation between fluorescence intensities and survivin DNA concentrations in the range of 0.005–20 nM. (D) Specificity of the survivin DNA initiated PER-based DNA machine.

Fig. 2