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. 2020 Nov 26;186:104990. doi: 10.1016/j.antiviral.2020.104990

Fig. 5.

Fig. 5

Intracellular calcium levels upon SERMs treatment. (A) Representative confocal micrographs of calcium imaging with Fluo8 in Vero cells pretreated with Verapamil, Raloxifene, or DMSO. (B) Representative images of ASFV B54-cherryFP infected cells after 16 h harboring the characteristic viral replication factories in controls. ASFV factories were absent in drug-treated cells. Bar 25 μm. (C) Time-response of intracellular Ca2+ detected with Fluo8. Each graph represented the average Ca2+ response to individual compounds in uninfected or infected cells. VER was included as a known positive responder that elevated intracellular Ca2+ levels. The graph shows fluorescence intensities in cells shown in (A) and (B), at the time points indicated. Error bars indicate S.D. from three independent experiments.