Table 2. PCR programs used for the amplification of COI, ITS-2, 28S rRNA and 18S rRNA.

	COI and ITS-2			28S rRNA			18S rRNA
Step	Cycles	Time [min.:sec.]	Temp. [°C]	Cycles	Time [min:sec]	Temp. [°C]	Cycles	Time [min:sec]	Temp. [°C]
initial denaturation	–	03:00	98	–	05:00	98	–	05:00	98
denaturation	40	00:15	98	40	00:30	98	40	00:30	98
annealing		00:30	50		01:00	50		01:00	60
extension		00:30	72		01:00	72		01:30	72
final extension	–	07:00	72	–	07:00	72	–	07:00	72