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. 2020 Oct 22;9(11):348. doi: 10.3390/biology9110348

Figure 6.

Figure 6

The PI3K-AKT signaling axis downstream of GLP-1R in the regulation of SH-SY5Y neuronal differentiation. The inhibitors of PI3K and MEK, LY294002 and U0126, respectively, were added to the medium, together with GLP-1, to assess the role of the GLP-1 signaling axis in the regulation of neuronal differentiation. Immunostaining images showed that LY294002 (a) inhibitor effectively suppressed neuronal differentiation and led to significant cell death (b). The U0126 inhibitor did not affect neuronal differentiation (a), although significant cell death was observed (b). The cell viability assay also has shown that LY294002 induced more cell death than U0126 (c). The results suggested that the PI3K-AKT-CREB axis is the predominant downstream signaling pathway regulating SH-SY5Y neuronal differentiation. (M ± SE; # p < 0.05; ### p < 0.001; compared to the value of GLP-1 control group, n = 3).