Table 3.

Prediction of protein instability from different software programs for the pathogenic variant, Cys66Tyr.

Software	Version	Prediction
MUpro [18]	1.1	Decrease stability
CUPSAT [19]	Release 2018.1	Destabilising
AUTO-MUTE [20]	2.0	Decrease stability
Site Directed Mutator (SDM) [21]	Server Access 02/21/2020	Reduced stability
I-Mutant2.0 [22]	2.0	Decrease
mCSM [23]	Server Access 02/21/2020	Destabilising