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. 2020 Oct 22;12(11):1005. doi: 10.3390/pharmaceutics12111005

Figure 1.

Figure 1

(A) Expression vector for pBABE-puro-hTERT [34] used to transfect Phoenix-AMPHO packaging cells for subsequent transduction into primary human SCs. Phase contrast microscopy images at 10× magnification of (B) primary human SCs at passage 4 and (C) immortalized hT-SerCs at passage 21. White arrows indicate tripartite nucleoli [41] within the nucleus of each cell. (D) Relative mRNA expression of human TERT assessed by RT-qPCR using the 2−ΔΔCt method. Ct values were normalized to GAPDH as the reference gene. (E) Representative Western blot of three independent experiments for the expression of human TERT in primary human SCs at passages 4 and 6 (lanes 1–3) and post-passage 20 hT-SerCs (lanes 4–6). The molecular weight ladder is denoted as L. Beta-actin served as the loading control.