Table 8.

Cycler conditions for species-specific PCR assays.

Target Species	Initial Denaturation	Denaturation	Annealing	Extension	No. of Cycles
F. temperatum	95 °C, 120 s	94 °C, 30 s	63 °C, 30 s	68 °C, 30 s	35
F. subglutinans	95 °C, 120 s	94 °C, 30 s	65 °C, 30 s	68 °C, 40 s	35
F. verticillioides	95 °C, 120 s	94 °C, 40 s	62.5 °C, 30 s	68 °C, 40 s	35
F. proliferatum	95 °C, 120 s	94 °C, 35 s	64 °C, 30 s	68 °C, 35 s	35