Figure 6.

Effect of HF diet supplemented with PA or LA on markers of non-alcoholic fatty liver disease (NAFLD). Liver sections (4 µm) from CD (A), HF + PA (B), and HF + LA-fed mice (C) were stained with hematoxylin and eosin. Pictures were taken at 20X magnification using a Nikon Eclipse 80i microscope. Histological grade for the degree of steatosis is shown (D). Quantification of liver triacylglycerol by gas chromatography (E). Liver weight measured at sacrifice is provided (F). Plasma levels of ALT and AST, markers of liver injury are shown (G,H). Values are expressed as mean ± SEM of 6–8 samples in each group. * p < 0.05, ^# p < 0.01, and ^ p < 0.001 versus CD. ALT, alanine aminotransferase; AST, aspartate aminotransferase; CD, chow diet; HF + PA, high fat + palmitic acid; HF + LA, high fat + lauric acid.