Figure 8.

Effect of HF diet supplemented with PA or LA on markers of hepatic inflammation. Immunohistochemical analysis was performed to detect MCP-1 in liver sections from CD (A), HF + PA (B), and HF + LA-fed mice (C). Representative images at 20× magnification are shown. The intensity of MCP-1 staining was quantified (D). Quantitative real-time PCR was performed for the mRNA levels of inflammatory genes such as Ccl2 and Tnfα (E,F). Values are expressed as mean ± SEM of 6–8 samples in each group. MCP-1, monocyte chemoattractant protein-1; Ccl2, C-C chemokine ligand 2; Tnfα, Tumor necrosis factor α; CD, chow diet; HF + PA, high fat + palmitic acid; HF + LA, high fat + lauric acid.