Table 1.

Chemical characterization of LJWE constituents using ultra-performance liquid chromatography-tandem mass spectrometry.

No	Rt (min)	Calculated (m/z)	Estimated (m/z)	Adduct	Error (ppm)	Formula	MS/MS Fragments (m/z)	Identifications
Amino acids
1	2.2	132.102	132.102	[M + H]+	2.683	C6H13NO2	86	L-Leucine/Isoleucine *
2	3.6	166.086	166.087	[M + H]+	2.284	C9H11NO2	166, 120	Phenylalanine *
3	4.8	205.097	205.098	[M + H]+	1.948	C11H12N2O2	188, 159, 146	L-Tryptophan *
Fatty acid derivatives
4	18.6	319.228	319.228	[M − H]−	1.736	C20H32O3	319, 301, 257, 219, 175, 113	15-Hydroxy-5,8,11,13 -eicosatetraenoic acid *
5	20.0	275.202	275.202	[M − H]−	2.033	C18H28O2	275, 231	Stearidonic acid *
6	20.6	301.217	301.218	[M − H]−	1.673	C20H30O2	301, 257, 203	Eicosapentaenoic acid *
7	20.7	277.217	277.218	[M − H]−	1.928	C18H30O2	277	Linolenic acid *
8	21.2	256.263	256.264	[M + H]+	1.305	C16H33NO	256, 116, 102, 88	Hexadecanamide
9	21.3	303.233	303.234	[M − H]−	2.083	C20H32O2	303, 259	Arachidonic acid *
10	21.4	282.279	282.280	[M + H]+	2.069	C18H35NO	282, 265, 247, 177, 149, 135	Oleamide
11	21.5	279.233	279.234	[M − H]−	1.934	C18H32O2	279	Linoleic acid *
12	21.9	284.294	284.295	[M + H]+	1.430	C18H37NO	284, 88	Stearamide
13	22.2	255.233	255.233	[M − H]−	1.458	C16H32O2	255	Palmitic acid *
14	23.3	283.264	283.265	[M + H]+	1.838	C18H36O2	283	Stearic acid *

Rt, retention time. * Compared with retention time (Rt) and the mass spectrum of reference standards.