Table 1.
The role of NNT-AS1 in different human cancers.
| Tumor Type | Expression Level | Number of Clinical Samples | Assessed Cell Lines | Effect In Vitro | Effect In Vivo | Regulatory Mechanism | Ref. |
|---|---|---|---|---|---|---|---|
| NSCLC | Up-regulated | 1037 tumor samples and 108 normal samples from TCGA database; 43 paired tissues | H1299, H23, A549, SPC-A-1, H522 | Proliferation ↑, migration and invasion ↑ | -- | NNT-AS1/miR-129-5p axis | [16] |
| Up-regulated | 37 paired tissues | H1650, PC-9, A549, H1299 | Proliferation ↑, migration and invasion ↑, EMT ↑ | Tumor growth ↑ | NNT-AS1/miR-22-3p/YAP1 axis | [17] | |
| Up-regulated | 10 paired tissues of DDP resistant and non-resistant | A549 and SPCA-1 of DDP resistant and non-resistant | DDP resistance ↑, proliferation ↑, colony formation ↑, cell cycle arrest ↓, apoptosis ↓ | -- | Activate MAPK/Slug signaling pathway | [18] | |
| Up-regulated | 31 paired tissues | H1299, H23, H522, A549 of DDP resistant and non-resistant | DDP resistance ↑, proliferation ↑, poptosis ↓, migration and invasion ↑ | -- | NNT-AS1/miR-1236-3p/ATG7 axis | [19] | |
| LUSC | Up-regulated | 46 paired tissues | Apoptosis ↓, migration and invasion ↑ | -- | NNT-AS1/miR-22/FOXM1 axis | [20] | |
| CRC | Up-regulated | 70 paired tissues | SW480, SW620 | Proliferation ↑, migration and invasion, colony formation ↑, cell cycle arrest ↑, EMT ↑ | Tumor growth ↑ Liver metastasis ↑ | Activate MAPK/Erk signaling pathway | [14] |
| Gastric cancer | Up-regulated | 48 paired tissues | BGC-823, MGC-803, AGS, SGC-7901, MKN-45 | Proliferation ↑, cell cycle arrest ↓, migration and invasion ↑ | Tumor growth ↑ | NNT-AS1/miR-424/E2F1 axis | [32] |
| Up-regulated | 40 paired tissues | HGC-27, MGC-803, MKN-45, SGC-7901 | Proliferation ↑, cell cycle arrest ↓, migration and invasion ↑ | -- | NNT-AS1/miR-363 axis | [33] | |
| Down-regulated | 30 paired tissues | -- | -- | -- | -- | [34] | |
| Up-regulated | 25 paired tissues | AGS, HGc-27 | Proliferation ↑, apoptosis ↓, migration and invasion ↑ | -- | miR-142-5p/SOX4/Wnt/β-catenin signaling pathway | [35] | |
| HCC | Up-regulated | 42 paired tissues | HepG2, Huh7 | Proliferation ↑, colony formation ↑, apoptosis ↓, cell cycle arrest ↓ | Tumor growth ↑ | NNT-AS1/miR-363/CDK6 axis | [21] |
| Cholangiocarci-noma | Up-regulated | 48 paired tissues | Huh28, KMBC, HCCC9810, HuCCT1, RBE, CCLP1, QBC-939 | Proliferation ↑, colony formation ↑, migration and invasion ↑ | Tumor growth ↑ | NNT-AS1/miR-485/BCL9 | [22] |
| Up-regulated | 20 paired tissues | SG231, CCLP1, HuCCT1, TFK1 | Proliferation ↑, EMT ↑ | -- | NNT-AS1/miR-203/IGF1R/ZEB1; activate PI3K/AKT and ERK1/2 pathways | [23] | |
| Up-regulated | Cohorts from TCGA database (number not shown); 30 paired tissues | RBE, HuCCT1, QBC939, TFK1 | Proliferation ↑, migration and invasion ↑, EMT ↑ | Tumor growth ↑ | NNT-AS1/miR-142-5p/HMGA2 axis | [24] | |
| Cervical cancer | Up-regulated | 79 paired tissues | SiHa, CaSki | Proliferation ↑, migration and invasion ↑, cell cycle arrest ↑, apoptosis ↓ | Tumor growth ↑ | Activate Wnt/β–catenin pathway | [25] |
| Up-regulated | 24 (chemo-sensitive) + 34 (chemo-resistant) paired tissues | HeLa, SiHa | DDP resistance ↑, proliferation ↑, Migration and invasion ↑, EMT ↑, apoptosis ↓ | DDP resistance ↑Tumor growth ↑ | NNT-AS1/miR-186/HMGB1 axis | [26] | |
| Breast cancer | Up-regulated | 64 paired tissues | MDA-MB-468, MCF-7, MD-MB-231 | Proliferation ↑, colony formation ↑, migration and invasion ↑, EMT ↑ | -- | NNT-AS1/miR-142-3p/ZEB1 axis | [36] |
| Down-regulated | 54 paired tissues | -- | -- | -- | -- | [37] | |
| Down-regulated * | 237 tumor samples and 11 normal samples from GEO datasets | -- | -- | -- | -- | [38] | |
| Ovarian cancer | Down-regulated | 55 paired tissues | HO-8910PM, HO-8910, OVCAR3, SK-OV-3 | Colony formation ↓, cell cycle arrest ↑, migration and invasion ↓, apoptosis ↑ | -- | -- | [39] |
| Osteosarcoma | Up-regulated | 126 paired tissues and 18 healthy controls | U2OS, Saos2, OS-9901, SOSP-9607, MG-63, OS-732 | Proliferation ↑, colony formation ↑, cell cycle arrest ↓, apoptosis ↓, migration and invasion ↑ | Tumor growth ↑ | AKT, C-myc, CyclinD1, BCL-2, MMP-2, MMP-9 | [27] |
| Up-regulated | -- | OS-732, U2OS | Proliferation ↑, colony formation ↑, apoptosis, migration and invasion ↑ | Tumor growth ↑ | NNT-AS1/miR-320a; Activate PI3K/AKT signaling pathway | [28] | |
| Bladder cancer | Up-regulated | -- | RT-4, UM-UC-3, J82, T24 | Proliferation ↑, colony formation ↑, migration and invasion ↑, EMT ↑, apoptosis ↓ | -- | NNT-AS1/miR-1301-3p/PODXL axis; activate Wnt pathway | [29] |
| Up-regulated | 47 paired tissues | T24, 5637, UM-UC-3, TCC-SUP | Proliferation ↑, migration and invasion, apoptosis ↓ | -- | NNT-AS1/miR-496/HMGB1 axis | [30] | |
| Glioma | Up-regulated | 73 paired tissues | U87, LN229, U251 | Proliferation, cell cycle arrest ↓, migration and invasion ↑, EMT ↑, apoptosis ↓ | -- | NNT-AS1/miR-494-3p/PRMT1 axis | [31] |
* Triple-negative breast cancer; NSCLC, non-small-cell lung cancer; LUSC, lung squamous cell carcinoma; CRC, colorectal cancer; HCC, hepatocellular carcinoma; TCGA, The Cancer Genome Atlas; GEO, Gene Expression Omnibus; EMT, epithelial–mesenchymal transition; DDP, cisplatin; ↑, promotion; ↓, inhibition.