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CD123 expression in various myeloid neoplasms, as assessed by flow cytometry and immunohistochemistry (CD123/TCF4 dual-color stain). In systemic mastocytosis, neoplastic mast cells express CD123 (red signal) without TCF4 (brown signal); a few admixed plasmacytoid dendritic cells with CD123/TCF4 coexpression can be seen (arrowhead). (Main figures at 200× magnification (inset 400×), hematoxylin counterstain).
