Figure 2.

MIE locus architecture during repressive and active states. Histone modifications and chromatin modifiers and readers associate with the entirety of the MIE region. On the other hand, transcription factors are recruited to the enhancer, unique, and modulator regions. Together, these factors regulate the balance of this locus between repressive (top) and active (bottom) states. During latency and quiescence, the MIE locus is repressed, while reactivation triggers the switch to a more active state, which is maintained during lytic replication. When repressed, the locus is in a closed chromatin conformation (top), which is relaxed and opened once activated (bottom). Chromatin modifiers and readers are noted for each state at the top right of each box. Transcription factors are shown below the locus and are color-coded based on the region to which they bind. Numbers next to the transcription factors denote the number of sites within that specific region. Those without a number have a single site within the designated region. AUG40 and AUG60 (blue asterisks) denote the start sites for IE40 and IE60, respectively. Canonical AUG noted by green asterisk.