Table 2.
List of identified glucosinolates, retention time (RT), calibration curves, and multiple reaction monitoring (MRM) conditions for quantitation of glucosinolates by negative ion MRM (see Supplementary File (Figure S1) for chromatogram).
| Glucosinolates | RT (min) | MRM Transition | CID (ev) | Dwell Time (sec) | Calibration Curve Parameters |
|---|---|---|---|---|---|
| Progoitrin (PRO) | 1.41 | 387.77 > 194.85 | 20 | 0.033 | Y = 3.59902X – 20.5808 (r2 = 0.999) |
| Gluconapin (GNA) | 3.02 | 371.74 > 258.74 | 20 | 0.033 | Y = 3.50074X + 3.51886 (r2 = 0.996) |
| Glucobrassicanapin (GBN) | 4.42 | 385.71 > 258.87 | 25 | 0.033 | Y = 2.68899X – 2.8434 (r2 = 0.994) |
| Glucotropaeolin (TRO) | 4.84 | 407.72 > 258.87 | 20 | 0.033 | Y = 6.27084X – 4.49552 (r2 = 0.999) |
| Glucoerucin (ERU) | 4.97 | 419.69 > 258.74 | 25 | 0.033 | Y = 2.41077X + 16.6315 (r2 = 0.999) |
| Glucobrassicin (GBC) | 5.61 | 446.69 > 204.94 | 20 | 0.033 | Y = 1.76969X – 11.3033 (r2 = 0.999) |
| Glucoberteroin (BER) | 6.29 | 433.72 > 275.06 | 20 | 0.033 | Y = 2.92616X – 3.54071 (r2 = 0.993) |
| Gluconasturtiin (NAS) | 6.33 | 421.69 > 274.87 | 25 | 0.033 | Y = 1.98894X + 1.81048 (r2 = 0.994) |
CID = collision-induced dissociation; LOQ = limit of quantification; Pol. = polarity.