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Overall stability ranking order of miRNAs. MiR-22-3p and miR-93-5p were first and second in ranking order, whereas miR-16-5p was the least stable.
| Ranking Order (Better-Good-Average) | |||||
|---|---|---|---|---|---|
| Method | 1 | 2 | 3 | 4 | 5 |
| Delta CT | miR-22-3p | let-7i | miR-93-5p | let-7a | miR-16 |
| BestKeeper | miR-93-5p | miR-22-3p | miR-16 | let-7i | let-7a |
| Normfinder | miR-22-3p | let-7i | miR-93-5p | let-7a | miR-16 |
| Genorm | miR-22-3p/miR-93-5p | let-7i | let-7a | miR-16 | |
| Recommended comprehensive ranking | miR-22-3p | miR-93-5p | let-7i | let-7a | miR-16 |
