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. 2020 Oct 24;18(11):527. doi: 10.3390/md18110527

Figure 2.

Figure 2

The effect of metabolites 1–3 on the expression of inflammatory markers in RAW 264.7 cells post 12, 24 and 48 h incubation with the indicated compound. (A) TNFα production was measured using ELISA in cell culture supernatants and (B) inducible nitric oxide synthase (iNOS) (C) Arginase 1 (D) MRC1 and (E) IRAK-M mRNA levels were identified using real time PCR. All treatments have been compared to carbowax 400 0.1% v/v + 0.01% ethanol treated cells in each timepoint indicated. Disulfide concentration used for 1 and 2 treatments was 15.62 μΜ and for 3 it was 31.25 μΜ. Statistical analysis was carried out using a Mann–Whitney unpaired t-test in Graphpad Prism 7.0 and graphs represent mean ± SEM and (* indicates p < 0.05, ** indicates p < 0.01, *** indicates p < 0.001 compared to carbowax 12 h, # indicates p < 0.05, ## indicates p < 0.01, ### indicates p < 0.001 compared to carbowax 24 h, ● indicates p < 0.05, ●● indicates p < 0.01, ●●● indicates p < 0.001 compared to carbowax 48 h).