Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Nov 24;218(3):e20201062. doi: 10.1084/jem.20201062

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2020 Lev et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms/). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

Figure 4. — Immune repertoire determined by next-generation sequencing for the SLP76-mutated patient. (A) Flow cytometry analysis of surface membrane expression of 24 different TRB variable gene families in the patient’s CD3 cells (black bars) compared with healthy controls (ctrl; white bars, n = 85, provided by the kit). One representative result out of two is shown. (B) Treemap representation of TRG, TRB, and B cell receptor (IGH) repertoire in PBMCs from the patient and two age-matched healthy controls. Each square represents a unique V to J joining, and the size of the square represents relative frequency within that sample. Two representative controls out of four are shown. (C and D) Quantification of the diversity and unevenness of the TRG, TRB, and IGH repertoire using the Shannon’s H index of diversity (C) and the Simpson’s D index of unevenness (D) in five healthy controls and in the SLP76-mutated patient.