Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference |
Identifiers | Additional information |
|---|---|---|---|---|
| Strain, strain background (S. minnesota) | Monophosphoryl Lipid A (MPLA) | Invivogen | Cat#: tlrl-mpla | Cell culture: 1 µg/mL |
| Genetic reagent (Mus musculus, male) | P2RX7-deficient mice (P2rx7-/-) |
Jackson laboratories | B6.129P2-P2rx7tm1Gab/J | In vivo mouse models and biological samples. RRID:IMSR_JAX:005576 |
| Antibody | Anti-MMR (rat monoclonal, clone MR5D3) | Acris antibodies | Cat#:SM1857P | WB (1:1000), RRID:AB_1611247 |
| Antibody | Anti-Cystatin B (rat monoclonal, clone 227818) | R&D Systems | Cat#: MAB1409 | WB (1:1000), RRID:AB_2086095 |
| Antibody | Anti-Cathepsin B (rat monoclonal, clone 173317) | R&D Systems | Cat#: MAB965 | WB (1:1000), RRID:AB_2086935 |
| Antibody | Anti-Peptidyl-prolyl cis-trans isomerase A (rabbit monoclonal) | Abcam | Cat#: ab41684 | WB (1:1000), RRID:AB_879768 |
| Sequence-based reagent | KiCqStart SYBR Green Primers | Sigma-Aldrich |
Tnfa
(NM_013693) Il-6 (NM_031168) Il1b (NM_008361) |
qRT-PCR |
| Peptide, recombinant protein | Human sCD14 recombinant protein | Preprotech | Cat#: 110–01 | In vivo: 10 µg/g RRID:AB_2877062 |
| Commercial assay, kit | ExoQuick-TC ULTRA EV isolation kit | System Biosciences (SBI) | Cat#: EQULTRA-20TC-1 | Extracellular vesicle isolation |
| Commercial assay, kit | Mouse CD14 DuoSet Elisa kit | R&D Systems | Cat#:DY982 | Detection of CD14 in biological fluids and culture supernatants. RRID:AB_2877065 |
| Commercial assay, kit | Magnetic Luminex Assay | R&D Systems | Cat#: LXSAMSM-15 |
Multiplex for mice serum |
| Chemical compound, drug | ATP | Sigma-Aldrich | A2383-5G | Cell culture: 3 mM For FACS: 5 mM |
| Chemical compound, drug | A438079 | Tocris | Cat#: 2972 | Cell culture: 10–20 µM In vivo: 100 µg/kg For FACS: 10 µM |
| Software, algorithm | NTA 3.1 software | Nano Sight Technology | NS300 instrument | Nanoparticle tracking analysis, RRID:SCR_014239 |