TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Description | Source or reference |
|---|---|---|
| Strains | ||
| E. coli | ||
| DH5α | F− ϕ80lacZΔM15 Δ(lacZYA-argF)U169 recA1 endA1 hsdR17 (rK−, mK+) phoA supE44 λ− thi-1 gyrA96 relA1 | 101 |
| P. aeruginosa | ||
| PA14 | Wild-type P. aeruginosa strain | 102 |
| ΔpqsA | pqsA clean deletion in PA14 background | Kind gift of Marvin Whiteley |
| ΔpqsH | pqsH clean deletion in PA14 background | 103 |
| ΔpqsE | pqsE clean deletion in PA14 background | This study |
| ΔpqsR | pqsR clean deletion in PA14 background | This study |
| Plasmids | ||
| pEX18gm | Gmr, suicide plasmid for gene replacement in P. aeruginosa | 95 |
| pEX18gm-pqsE | Gmr, pEX18gm-derived vector for clean-deletion of pqsE | This study |
| pEX18gm-pqsR | Gmr, pEX18gm-derived vector for clean-deletion of pqsR | This study |
| pJN105 | Gmr, araC-pBAD expression vector | 96 |
| pJN105-pqsA | Gmr, pJN105-derived pqsA overexpression vector | 55 |
| pJN105-pqsH | Gmr, pJN105-derived pqsH overexpression vector | This study |
| pJN105-pqsR | Gmr, pJN105-derived pqsR overexpression vector | This study |
| pCR 2.1 | Ampr, Kanr, TA-cloning vector | Invitrogen |
| pRK2013 | Kmr, helper plasmid used for triparental mating | 104 |