Skip to main content
. 2020 Nov 19;2020:2963540. doi: 10.1155/2020/2963540

Figure 6.

Figure 6

EA targeted microglial NLRP3 inflammasome to produce DA neuroprotection. Microglia-conditioned medium (MCM) prepared from BV-2 cell cultures with administration of EA (MCM (EA)), LPS (MCM (LPS)), LPS+EA (MCM (LPS+EA)), NLRP3 siRNA (MCM (NLRP3 siRNA)), NLRP3 siRNA+EA (MCM (NLRP3 siRNA+EA)), NLRP3 siRNA+LPS (MCM (NLRP3 siRNA+LPS)), and LPS+NLRP3 siRNA+EA (MCM (LPS+NLRP3 siRNA+EA)) was harvested and added to MN9D cells incubated for 24 h. MN9D cell viability was determined by MTT assay (a). TH protein expression was tested by western blot assay (b). Data were the mean ± SEM from three independent experiments performed in triplicate. ∗p < 0.05 compared with control cultures; #p < 0.05 compared with MCM (LPS)-treated cultures.