Figure 4.

RNA-Targeting Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) Systems for Coronavirus Inhibition.

The adeno-associated virus (AAV) serves as a carrier to deliver vector constructs consisting of both Cas13d effector and guide RNAs (gRNAs) (1). Once inside the cell, Cas13d protein and gRNA are expressed and Cas13d protein forms a complex with gRNA (2). The spacer sequence acts as a guide to the Cas13d effector by matching to the complementary sequences in the viral RNA genome (3), thus allowing the associated Cas13d effector to cleave the viral RNA (4), therefore disrupting viral functions. Created with BioRender.