Figure 5.

Identification of SP1, ROCK1, and ROCK2 as Target Genes of miR-135a-5p

Potential target sites of miR-135a-5p on the mRNA 3′ UTRs and several point mutations were generated at the binding site. A luciferase reporter assay identified the predicted binding sequence required for miR-135a-5p-mediated inhibition. (A) SP1 luciferase assay (n = 3). (B) ROCK1 luciferase assay (n = 3). (C) ROCK2 luciferase assay (n = 3). Statistical data were measurement data, described as mean ± standard deviation. ∗∗∗p < 0.001. NC, negative control.