. 2020 Aug 25;22:1051–1062. doi: 10.1016/j.omtn.2020.08.018

Table 3.

The Sequence of Primers, RNA Oligo, AgomiRs, and AntagomiRs in This Study

	Sequence (5′ to 3′)
Primers

hsa-miR-22-3p	forward: CACCGACAGTTCTTCAACTGGCAGCTT
hsa-miR-22-3p	reverse: AAAAAAGCTGCCAGTTGAAGAACTGTC
U6b	forward: CTCGCTTCGGCAGCACA
U6b	reverse: AACGCTTCACGAAYYYGCGT
MAPK14	forward: GGGACCTCCTTATAGATGATGAGTGG
MAPK14	reverse: GGACTCCATCTCTTCTTGGTCA
GAPDH	forward: CAGGCATAATGGTTAAAGTTGGTA
GAPDH	reverse: CATGTAATCAAGGTCAATGAATGG

RNA Oligo

miR-22 mimics	AAGCUGCCAGUUGAAGAACUGU
miR-22 mimics	ACAGUUCUUCAACUGGCAGCUU
miR-22 mimics NC	GCAACCUGUUAGACUAUGGAGA
miR-22 mimics NC	UCUCCAUAGUCUAACAGGUUGC
MAPK14	GUCCAUCAUUCAUGCGAAATT
MAPK14	UUUCGCAUGAAUGAUGGACTT
Negative control	UUCUCCGAACGUGUCACGUTT
Negative control	ACGUGACACGUUCGGAGAATT
GAPDH positive control	UGACCUCAACUACAUGGUUTT
GAPDH positive control	AACCAUGUAGUUGAGGUCATT

AntagomiRs and AgomiRs

mmu-miR-22-3p agomiR	AAGCUGCCAGUUGAAGAA CUGUAGUUCUUCAACUGG CAGCUUUU
agomiR NC	UUCUCCGAACGUGUCAC GUTTACGUGACACGUUC GGAGAATT
mmu-miR-22-3p antagomiR	ACAGUUCUUCAACUGGCAGCUU
antagomiR NC	CAGUACUUUUGUGUAGUACAA